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gfap promoter  (Addgene inc)


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    Addgene inc gfap promoter
    Gfap Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfap promoter/product/Addgene inc
    Average 93 stars, based on 17 article reviews
    gfap promoter - by Bioz Stars, 2026-06
    93/100 stars

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    (A) 2D, raster scan image showing vasculature labelled with Texas-Red and astrocytes expressing GCaMP6f. Annotated regions are soma (red), endfeet (blue), and process (magenta). (B) Representative measurements of spontaneous Ca 2+ release in cortical astrocytes cell compartments recorded for 10 mins. (C) Amplitude, Frequency and Duration of Ca 2+ spikes at different astrocyte ROIs. (D) OISI measurement of cerebral hemodynamics in response to 3s whisker stimulation in left barrel cortex in WT and AD mice. (E) Representative two-photon raster scan images of diving arteriole dilation and astrocyte Ca 2+ release during whisker stimulation trials. (F-I): Trial-averaged profiles of arteriole dilation and astrocyte Ca 2+ release in response to 3s whisker stimulation and quantification of the peak response. (J-L): Trial-averaged profiles of arteriole dilation and astrocyte Ca 2+ release in response to 30s whisker stimulation and the quantification of the peak response. Difference between WT and AD were tested using rank-sum test.

    Journal: bioRxiv

    Article Title: Systemic inflammation reduces astrocyte Ca 2+ and neurovascular coupling in a mouse model of Alzheimer’s disease

    doi: 10.1101/2025.08.31.673380

    Figure Lengend Snippet: (A) 2D, raster scan image showing vasculature labelled with Texas-Red and astrocytes expressing GCaMP6f. Annotated regions are soma (red), endfeet (blue), and process (magenta). (B) Representative measurements of spontaneous Ca 2+ release in cortical astrocytes cell compartments recorded for 10 mins. (C) Amplitude, Frequency and Duration of Ca 2+ spikes at different astrocyte ROIs. (D) OISI measurement of cerebral hemodynamics in response to 3s whisker stimulation in left barrel cortex in WT and AD mice. (E) Representative two-photon raster scan images of diving arteriole dilation and astrocyte Ca 2+ release during whisker stimulation trials. (F-I): Trial-averaged profiles of arteriole dilation and astrocyte Ca 2+ release in response to 3s whisker stimulation and quantification of the peak response. (J-L): Trial-averaged profiles of arteriole dilation and astrocyte Ca 2+ release in response to 30s whisker stimulation and the quantification of the peak response. Difference between WT and AD were tested using rank-sum test.

    Article Snippet: After the localization of the injection site, a ∼ 3 mm diameter of cranial window was created on the barrel cortex of the mouse, followed by targeted injection of a GFAP-promoted GCaMP6f virus (pZac2.1gfaABC1D-cyto-GCaMP6f, Plasmid #52925, Addgene).

    Techniques: Expressing, Whisker Assay